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Table 1 Mitochondrial, chloroplast, and nuclear genes derived probes, and associated primer sequences used for the characterization of the CinS2S2 and CinS1S4 libraries.

From: Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

Probea

Description

Accession numbersb

Primer sequences (5' → 3')

Tm (°C)

Size (bp)

atpA

F1-F0 ATP synthase, subunit alpha

X80469, X51422, AF034118, X52838

L: gatcttgtcaagcgcactgg

R: agtaatgcctgagtcgcagc

66

64

1000

atp9

F1-F0 ATP synthase, subunit 9

X51895, DQ539624

L: aataggggccggagctgc

R: gaaaggccatcattggggc

69

69

184

cob

Apocytochrome b

X98362, EF674047, EF674014, DQ916732

L: gagttatagcagtcctaggg

R: ctagtagtaagcaatccgcc

55

58

697

cox2

Cytochrome c oxydase, subunit 2

AJ414385, EF547230, DQ004553, EF488904

L: atttcaagacgcagcaacacc

R: gtactacctcgtccattgag

65

56

264

matK

Maturase K

AJ633132

L: tggttcaggctcttcgctattgg

R: cgtcccttttgaagcaagaattg

70

68

398

ndhF

NADH dehydrogenase

AY504736

L: tacttgtattgattctatttctttg

R: caacaagattaaagattaaaaaag

55

55

581

rbcL

Ribulose 1,5-biphosphate carboxylase/oxygenase, large subunit

L13652

L: ttgccgagataatggcctac

R: ccaaagatctcggtcagagc

64

64

336

trnL-trnF

Intergenic spacer tRNA-Leu (trnL)-tRNA-Phe (trnF) genes

FJ490769

L: ggttcaagtccctctatcccc

R: ctaccagctgagctatcccg

65

64

397

CiAGP

Arabinogalactan protein

DT212458

L: aaaccaaccaagactttgaccacg

R: cccccttaagtttccacaaattac

58

64

418

CiSTM

SHOOT MERSISTEMLESS transcription factor

GU189066

L: gtaggtacatcatgtttgatggggtttggag

R: ccctaccttttggcaattca

73

64

344

  1. aatpA, atp9, cob and cox2 correspond to mitochondrial sequences, matK, ndhF, rbcL and trnL-trnF to chloroplast sequences, and CiAGP and CiSTM to cDNA sequences.bMultiple accession numbers for a probe indicate that primer pairs were designed from the consensus sequence of these accessions.