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Figure 1 | BMC Research Notes

Figure 1

From: Assessing the efficiency and significance of Methylated DNA Immunoprecipitation (MeDIP) assays in using in vitro methylated genomic DNA

Figure 1

Validation of MeDIP assay using in vitro methylated DNA. DNA samples from the human cell line SilALL were methylated in vitro (+ M.SssI) or not, and subjected to MeDIP assay. Relative enrichment at normally methylated (PCDHGA12 and RPIB9) or unmethylated (ACTB and GAPDH) gene promoters was analyzed by real-time quantitative PCR. Both the PCDHGA12 and RPIB9 gene promoters were previously found to be commonly methylated in T-ALLs and derived cell lines [27]. Results shown represent the mean values of triplicate PCRs; and are representative of three independent experiments.

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