Development of polyclonal antiserum against E2 and detection of SAVH20/03 by IFAT. (A) A region of E2 was identified based on alignment of SAV3 with antigenic regions of the SINV and RRV E2 proteins. The peptide corresponded to amino acids 184 to 236 of the putative SAV3 E2 protein was cloned and expressed in E. coli, and used for immunization of a rabbit. (B) IFAT using the obtained E2 antiserum on CHSE cells infected with SAVH20/03 demonstrated specific staining located to the plasma membrane and cytoplasmic structures, possibly ER and Golgi (arrow). No staining could be observed in mock treated cells.