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Table 3 Tissue culture infective dose (TCID50/ml) produced by wild type SAVH20/03 and different mutants 7 d.p.i. in CHSE cells.

From: Development of infectious cDNA clones of Salmonid alphavirus subtype 3

Virus

TCID50/ml

SAVH20/03

1.6 × 107

rSAVE2206S

2.8 × 106

rSAVE2206P

1.6 × 107

rSAVnsP2197DE2206S

< 10

rSAVnsP2197DE2206P

< 10