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Figure 1 | BMC Research Notes

Figure 1

From: The RAD51 and DMC1 homoeologous genes of bread wheat: cloning, molecular characterization and expression analysis

Figure 1

PCR assay for the chromosomal localization of the TaRAD51 and TaDMC1 homoeologous loci, respectively, on group 7 and group 5 of hexaploid wheat. Each primer set designed for TaRAD51 was used to amplify NT7A7B, NT7A7D, NT7B7A, NT7B7D, NT7D7A, NT7D7B, water control and CS. (a) TaRAD51 (A) and (D) genome-specific primer PCR amplifications. The two bands (indicated by red arrows) in Nullisomic 7B and tetrasomic for 7A and 7D lanes can be allocated either to A or D genomes based on PCR amplification on other Nullisomics (Nullisomics 7A and 7D); TaRAD51(B) genome-specific primer PCR amplification and absence of bands in the line nullisomic for 7B and tetrasomic for both 7A and 7D allocates this primer to genome 7B. Each primer set for TaDMC1 was used to amplify NT5A5B, NT5B5A, NT5D5A, water control and CS (b) TaDMC1(A), (B) & (D) genome-specific primer PCR amplification. Absence of bands in the group 5 Nullisomics allocates the primer to the respective genomes; M = 2-log ladder (NEB). Only group 5 and group 7 of Nulli-tetrasomics are shown.

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