Alignment of 3'- and 5'-UTR sequences of TaRAD51 and TaDMC1 homoeologues from hexaploid wheat indicating the location of genome specific primers. (a) TaRAD51 cDNA genome-specific primer design. (b) TaDMC1 cDNA genome-specific primer design. Deletions are shown by dashes. The positions of the 3' and 5' UTRs in relation to the start codon 'A' are indicated at the top of the sequence. The sequence of the forward primer and complementary sequences of the reverse primers are shown in bold. The black arrows indicate the position of forward and reverse primers.