Figure 1From: A novel real-time PCR assay for specific detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk with the inherent possibility of differentiation between viable and dead cellsAmplification plot (A) and standard curve (B) of the optimized real-time PCR assay for the Mptb52.16 target. Based on the molecular weight of the genome of MAP strain K-10 (GenBank accession number AE016958), 1 ng DNA equals 9.59 × 105 copies of the entire genome. The MAP-specific target Mptb52.16 is a single-copy gene. Thus, this figure equals the number of PCR targets per ng. The dilution series ranged from 9.59 × 105 to 9.59 × 100 copies of the genomic DNA from MAP strain CIP 103974 per PCR. Fifty copies of an IAC were added to each reaction. The MAP-specific probe was detected in the FAM channel, whereas the IAC-specific probe was detected in the HEX channel.Back to article page