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Table 1 Bacterial strains used for inclusivity and exclusivity testing of the Mptb52.16 real-time PCR assay

From: A novel real-time PCR assay for specific detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk with the inherent possibility of differentiation between viable and dead cells

Genus and species Source
Mycobacterium avium subsp. paratuberculosis CIPa 103964
Mycobacterium avium subsp. paratuberculosis CIP 103967
Mycobacterium avium subsp. paratuberculosis CIP 103968
Mycobacterium avium subsp. paratuberculosis CIP 103971
Mycobacterium avium subsp. paratuberculosis CIP 103972
Mycobacterium avium subsp. paratuberculosis CIP 103973
Mycobacterium avium subsp. paratuberculosis CIP 103974
Mycobacterium avium subsp. paratuberculosis CIP 103975
Mycobacterium avium subsp. paratuberculosis CIP 103976
Mycobacterium avium subsp. paratuberculosis CIP 103977
Mycobacterium abscessus DSMb 44196
Mycobacterium aurum DSM 43999
Mycobacterium avium subsp.avium DSM 44156
Mycobacterium avium subsp.silvaticum DSM 44175
Mycobacterium chitae DSM 44633
Mycobacterium duvalii DSM 44244
Mycobacterium flavescens DSM 43991
Mycobacterium fortuitum subsp. fortuitum DSM 46621
Mycobacterium gordonae DSM 44160
Mycobacterium intracellulare DSM 43223
Mycobacterium kansasii DSM 44162
Mycobacterium marinum DSM 44344
Mycobacterium neoaurum DSM 44074
Mycobacterium nonchromogenicum DSM 44164
Mycobacterium parafortuitum DSM 43528
Mycobacterium phlei DSM 43239
Mycobacterium scrofulaceum DSM 43992
Mycobacterium shiumoidei DSM 44152
Mycobacterium thermoresistibile DSM 44167
Mycobacterium triviale DSM 44153
Mycobacterium vaccae DSM 43292
Mycobacterium xenopi DSM 43995
Bacillus cereus NCTCc 7464
Clostridium bovis DSM 20582
Clostridium pyogenes DSM 20630
Enterococcus faecalis ATCCd 19433
Escherichia coli NCTC 9001
Lactococcus lactis DSM 20069
Listeria monocytogenes NCTC 11994
Pseudomonas aeruginosa NCTC 10662
Salmonella typhimurium ATCC 14028
Staphylococcus aureus NCTC 1803
Staphylococcus epidermidis ATCC 12228
Streptococcus agalactiae DSM 2134
Streptococcus dysgalactiae subsp. dysgalactiae DSM 20662
Streptococcus dysgalactiae subsp. equisimilis DSM 6176
Streptococcus thermophilus DSM 20617
Streptococcus uberis DSM 20569
  1. Colonies (MAP and non-MAP mycobacteria) suspended in 1 ml of Ringer's solution or overnight broth cultures (raw milk microflora) were subjected to DNA isolation using the NucleoSpin tissue kit (Machery-Nagel, Düren, Germany). The DNA concentration was measured fluorimetrically using a HoeferDyNA Quant200 apparatus (Pharmacia Biotech, San Francisco, USA) and the DNA was diluted to 0.1 ng/μl ddH2O.
  2. Notes: a CIP: Institute Pasteur, b DSM: German Collection of Microorgansims, c NCTC: National Collection of Type Cultures, d ATCC: American Type Culture Collection