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Table 2 Primers and probes used for real-time PCR

From: A novel real-time PCR assay for specific detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk with the inherent possibility of differentiation between viable and dead cells

Target Oligonucleotide Function
Mptb52.1 5'-GCT CGC CGT GAT GTT GTT G-3' forward primer
  5'-FAM-CTT GAC TCA GAT GCG GTG GAT GGA-BHQ1-3' probe
  5'-CCC GAA AGC CCT TCT CAA G-3'rr reverse primer
Mptb52.16 5'-CGA CAC CCC TCC AAT TGA TC-3' forward primer
  5'-FAM-TTC CGC ACC CCT GAT GGA GTG T-BHQ1-3' probe
  5'-ACC CGG AAG ATT GTC ACC G-3' reverse primer
Mptb54.33 5'-CTC CTT CCA CGT CAG AAG CC-3' forward primer
  5'-FAM-TTA CCA GTC ATC GGA GCC AGG TCG-BHQ1-3' probe
  5'-GGA CGA CAC CAC TTG AAG AGC-3' reverse primer
IAC 5'-TCA CAG CCC TCC AAC TAT CAG AA-3' forward primer
  5'-HEX-TTC GTA GCC ACT GGG TTC CAC GG-BHQ1-3' probe
  5'-TGT TGT CCC AAT CAT CAC GTG TA-3' reverse primer