Analysis of the exon-intron structure of the CHD7 CRA_e novel transcript. (A) In the upper part of the figure the exon-intron structure of the canonical CHD7 gene (accession number NM_017780.2) is represented. Exons 4 to 35 have been omitted for clarity. In the lower part the exon-intron structure of the CHD7 CRA_e alternative transcript deduced from sequencing and annealing to the canonical transcript sequence is represented. Splicing is represented as bold lines and numbers below boxes indicate the exon numbers. Rectangles represent the exons, with 0.25 cm in length being equivalent to 100 nucleotides, and the bold line represents the introns, with 0.5 cm being equivalent to 10 kilobase pairs. Regions of alternatively spliced exons 3 and 36 are lightly shaded. (B) Comparative alignment of nucleotide sequences around exonics splicing sites at exons 3 and 36 of human CHD7 with the corresponding exonics regions in Chimpanzee, Horse, Dog, Mouse, short-tailed Opossum, Rat, Boar, Cattle and Chicken. The splicing donor (SD), putative branch point (BP) and splicing acceptor (SA) sites are boxed. The pyrimidine-rich region is shown in bold. Nucleotides differing from the human sequence are underlined.