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Figure 4 | BMC Research Notes

Figure 4

From: Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase

Figure 4

RT-PCR analysis of the CHD7 CRA_e transcript expression in normal human tissue total RNA samples. To detect the novel CHD7 transcript by RT-PCR we used primers adjacent to the alternative splice site which generate a DNA fragment of approximately 2 kbp. As internal control of the reaction we performed RT-PCR of the same samples using primers specific for the NOTCH2 transcript which yield a DNA fragment of approximately 300 bp (lower part of the figure). A negative control without cDNA was run with each reaction.

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