Figure 3

Organ-specific expression of selected marker genes. (A) qPCR transcript levels are presented as bars (relative expression levels shown on the primary axis were normalized to TIM44-2), while corresponding log₂ expression values of ATH1 GeneChips are displayed as curves on the secondary axis. ALC is expressed in the valves margins of the silique [39]. RD20 is expressed specifically in siliques, but not in seeds [40]. ATS3 expression is seed-specific [38]. The presented genes are useful markers for separated A. thaliana silique samples (isolated valve and replum organs, isolated seeds). (B) ATH1 GeneChip hybridization data were used to determine the number of differentially regulated genes at each developmental stage normalized to stage 10. The number of significantly up- and down-regulated genes is shown (log₂ foldchange ≥1.0 or log₂ foldchange≤-1.0, p ≤ 0.05). Color key: blue, siliques containing seeds; green, isolated valves and replum organs; red, isolated seeds. Abbreviations are as follows: ALC alcatraz, RD20 responsive to dehydration 20, ATS3 A. thaliana seed gene3, TIM44-2 translocase inner membrane subunit 44-2.