MDA | S. aur gDNA | Probe | Average Ct | SEM (±) |
|---|
N | 100 ng | proS | 17.8 | 0.03 |
N | 10 ng | proS | 22.1 | 0.05 |
N | 1 ng | proS | 24.6 | 0.03 |
N | 0.1 ng | proS | 27.7 | 0.06 |
N | 0.01 ng | proS | 31.7 | 0.26 |
N | 0 ng | proS | U | U |
(N) | 0.01 ng | proS | 35.4 | 0.85 |
(Y) | 0.01 ng | proS | 16.9 | 0.05 |
- Triplicate samples of isolated S. aureus genomic DNA were assayed by qPCR; the calculated average threshold critical cycle (Ct) values are provided, as is the standard error. Each sample concentration gave a highly reproducible result; the data shown are using the proS probe, but equivalent results were obtained using lysS. Pre-treatment of S. aureus gDNA by MDA resulted in a massive reduction in Ct, indicating a massive amplification of template. In MDA control lacking the DNA polyermase, Ct actually slightly increased, due to dilution of the starting material. 0 ng of gDNA (negative control) gave no detectable signal (Ct undetermined, represented as "U" above).
