Triplicate samples of isolated S. aureus genomic DNA were assayed by qPCR; the calculated average threshold critical cycle (Ct) values are provided, as is the standard error. Each sample concentration gave a highly reproducible result; the data shown are using the proS probe, but equivalent results were obtained using lysS. Pre-treatment of S. aureus gDNA by MDA resulted in a massive reduction in Ct, indicating a massive amplification of template. In MDA control lacking the DNA polyermase, Ct actually slightly increased, due to dilution of the starting material. 0 ng of gDNA (negative control) gave no detectable signal (Ct undetermined, represented as "U" above).