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Table 3 qPCR assay of aspirated synovial fluid, +/- lysis and MDA

From: Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluid

Sample vol. Probe Lysis -, MDA - Lysis +, MDA - Lysis +, MDA +
0.1 μl proS U + +
1.0 μl proS U U +
10 μl proS U U +
0.1 μl proS U + +
1.0 μl proS U U +
10 μl proS U U +
0.1 μl lysS U + +
1.0 μl lysS U U +
10 μl lysS U U +
0.1 μl lysS U + +
1.0 μl lysS U U +
10 μl lysS U U +
  1. Varying volumes of joint aspirate were treated as per the conditions listed atop each column, either with or without lysis and MDA. "U" indicates that no critical cycle value was obtained (undetermined), and thus that no template DNA was detected. "+" indicates that a valid Ct value was obtained, and an appropriate sigmoidal accumulation curve, indicating successful detection of template. Results were identical in two samples of aspirate, using the proS and lysS probes.
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