EST library selection. The screenshot shows the MediPlEx web page to select the genes of interest by using the EST library assembly. For each library, the user can select one of the three states: 'MUST contain ESTs', 'MUST NOT contain ESTs', and 'MAY contain ESTs'. 'MUST contain ESTs' means that the resulting TCs have to consist of at least one EST from these libraries. 'MUST NOT contain ESTs' denotes that the resulting TCs are not allowed to have an EST assembled from these libraries at all, and 'MAY contain ESTs' indicates that it does not matter if an EST is assembled from these libraries to a TC or not (see venn diagram). EST library preselection are implemented via buttons, the complete library list is available on the bottom of the page. The EST library list is shortened for this screenshot.