Skip to main content
Figure 4 | BMC Research Notes

Figure 4

From: Allelic replacement of the streptococcal cysteine protease SpeB in a Δsrv mutant background restores biofilm formation

Figure 4

Addition of purified active SpeB inhibits biofilm formation. MGAS5005, MGAS5005ΔspeB and MGAS5005Δsrv ΔspeB were either untreated or treated with 1 μg/mL of purified SpeB (Toxin Technology, Inc., Sarasota, FL) 3 times at time 0, 6 h, and 12 h. Biofilm was measured at 18 h using CV staining as previously discussed. The level of reduction in biofilm formation was statistically significant ((***) P < 0.0001) compared to the untreated samples. MGAS5005Δsrv, with constitutive production of SpeB, is presented for comparison.

Back to article page