Figure 1

Transgene expression after use of SB100x transposase. Embryos injected with transgene pT2-mitfa- amyloidβ-GFP and SB100x mRNA were examined at 24 hpf for transgene expression by whole mount in situ transcript hybridisation against GFP sequences included in the transcript. A-D, lateral views of trunk region of embryos. E, dorsolateral view of hindbrain/spinal cord region of embryo. White arrowheads indicate some of the cells apparently expressing the transgene in a pattern consistent with expression in future melanophores in which the mitfa promoter is active. Size bars indicate approximately 100 uM. Deformities caused by the SB100 transposase mean that some structures (e.g. the yolk extension) are an inconsistent size. F, Histogram showing percentage rates of transient transgenesis driven by SB10 and SB100 transposases.