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Table 1 Results of injections of transposase mRNAs alone or with the mitfa-amyloidβ-GFP transgene

From: A hyperactive sleeping beauty transposase enhances transgenesis in zebrafish embryos

Injection

Normal % (n)

Mild % (n)

Severe % (n)

Uninjected

98 (59)

2 (1)

0

mitfa- amyloidβ-GFP only

94 (60)

3 (2)

3 (2)

   -with SB100 mRNA

62 (36)

28 (16)

10 (6)

   -with SB10 mRNA

97 (59)

3 (2)

0

SB100 mRNA only

40 (20)

16 (8)

44 (22)

SB10 mRNA only

83 (39)

8.5 (4)

8.5 (4)

TOL2 mRNA only

83 (38)

8.5 (4)

8.5 (4)

  1. Percentage of embryos at 24 hpf with the number of embryos observed in parentheses.
  2. Normal: wild type appearance. Mild: trunk/somitogenesis-like defects. Severe: epiboly-like defects.