TY - JOUR AU - Horspool, Daniel R. AU - Coope, Robin JN AU - Holt, Robert A. PY - 2010 DA - 2010/11/09 TI - Efficient assembly of very short oligonucleotides using T4 DNA Ligase JO - BMC Research Notes SP - 291 VL - 3 IS - 1 AB - In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short oligonucleotide substrates and defined conditions suitable for assembly of a plurality of oligonucleotides. SN - 1756-0500 UR - https://doi.org/10.1186/1756-0500-3-291 DO - 10.1186/1756-0500-3-291 ID - Horspool2010 ER -