Figure 4

Validation of DNA synthesis by ligation of octamers.

(a) PCR amplification of construct using 2 μL of the immobilized DNA product. The forward primer was identical to a region in the adaptor DNA attached to the first bead and the reverse was complementary to the tail end of the final intermediate to be ligated. (b) Electropherogram of a successfully cloned and sequenced product. The first 42-bp represent those of the adaptor while the latter 128-bp are those of the target fragment assembled with octamers.