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Figure 1 | BMC Research Notes

Figure 1

From: Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase

Figure 1

Transmembrane protein topology of Botrytis cinerea CHS3a. (A) Hydropathy profile of BcCHS3a based on the protein sequence, determined on http://mobyle.pasteur.fr web site by using the "Toppred" program. Hydrophobicity values above the cutoff correspond to potential transmembrane domains. Results supposed eight transmembrane domains at the C-terminal region of the protein. (B) Schematic representation of wild type CHS3a and recombinant proteins fragments. Predicted transmembrane domains and conserved sequence motifs in BcCHS3a obtained after HCA plots of BcCHS3a sequence with other non processive glycosyltransferase sequences [5]. CHS3a-SGC-423 (143-565) is indicated in black and CHS3a-SGC-359 (164-522) in grey below the graph.

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