Figure 2
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of protein samples from E. coli BL21(DE3) cells transformed by pet28-CHS3a-SGC-423 (A) or pet30-CHS3a-SGC-359 (B) plasmids. Aliquots of successive steps of purification : (A) CHS3a-SGC-423 Lanes : 1; whole cell fraction after solubilization of inclusion bodies; 2, molecular weight markers; 3, Ni2+-NTA-agarose column; 4, carboxymethyl-agarose column. (B) CHS3a-SGC-359 Lanes 1, molecular weight markers; 2 and 3, first and second Ni2+-NTA-agarose columns, respectively. The electrophoresis was performed in 10% PAGE and gels were stained by Coomassie blue.