Figure 3

Subcellular localization in epidermal onion cells of fusions of GFP with full-length CrMYC2, with different putative nuclear localization signals and with deletion mutants of CrMYC2. The green fluorescence of the GFP protein was visualized by epifluorescence microscopy. A: control with GFP alone. B: control with GFP fused to the NLS of the SV40 large T antigen. C: control with GFP fused to an endoplasmic reticulum retention signal. D: right, GFP fused to full-length CrMYC2, left: corresponding DAPI staining,. E-H: GFP fused with different putative NLS sequences from CrMYC2, 1, 2, 3 and 4, respectively; I-P: GFP fused with different deletion mutants of CrMYC2 (F1 to F8).