Table 1 Oligonucleotides used for the amplification of full-length or deletion mutants of Crmyc2 or for the reconstitution of sequences encoding the putative NLSs of the CrMYC2 protein
Fusion protein name | Nucleotide sequences of primers used to amplify the Crmyc2 fragments or to reconstitute the short coding sequences of the NLSs | |
|---|---|---|
CrMYC2 | forward | 5'-CCGCTCGAG ATGACGGACTAT-3' |
reverse | 5'-CCGCTCGAG TCATACCAAGAG-3' | |
F1 | forward | 5'-CCGCCATGG GCACCGCCGATGATGCA-3' |
reverse | 5'-CCGCCATGG GTACCAAGAGCCTCATCG-3' | |
F2 | forward | 5'-CCGCCATGG GGGTAGTTTTGCCCTCTACT- |
reverse | 5'-CCGACTAGT TACCAAGAGCCTCATCGAGT-3' | |
F3 | forward | 5'-CCGCCATGG GAAGGGAAGAGCCATTGAAT-3' |
reverse | 5'-CCGACTAGT TACCAAGAGCCTCATCGAGT-3' | |
F4 | forward | 5'-CCGCCATGG GGGTAGTTTTGCCCTCTACT-3' |
reverse | 5'-CCGCCATGG CCATTTGCAGGCTTTCTCCC 3' | |
F5 | forward | 5'-CCGCCATGG GAAGGGAAGAGCCATTGAAT-3' |
reverse | 5'-CCGACTAGT TAATTCAAGATCAAGAT-3' | |
F6 | forward | 5'-CCGCCATGG GAAGGGAAGAGCCATTGAAT-3' |
reverse | 5'-CCGACTAGT ATCAATATCCATATCCAAGTTACC-3' | |
F7 | forward | 5'-CCGCCATGG GTAACTTGGATATGGATATTG-3' |
reverse | 5'-CCGACTAGT TACCAAGAGCCTCATCGAGT-3' | |
F8 | forward | 5'-CCGCCATGG CTAAACTTCAA-3' |
reverse | 5'-CCGACTAGT TAATTCAAGATCAAGAT-3' | |
NLS1 | forward | 5'-CATGG GGAAGAGGAAGAATTCGTCTTCCGCGAGT TCTTTTGCAGAACAGGAACACAGAAAGAAAA-3' |
reverse | 5'-CTAGT TTTCTTTCTGTGTTCCTGTTCTGCAAAAGAA CTCGCGGAAGACGAATTCTTCCTCTTCCC-3' | |
NLS2 | forward | 5'-CATGG AGAACAAGAACAAGAAAAGGCCATCTA-3' |
reverse | 5'-CTAGT AGATGGCCTTTTCTTGTTCTTGTTCTC-3' | |
NLS3 | forward | 5'-CATGG AGAAACGGCCTAGGAAGAGGA-3' |
reverse | 5'-CTAGT CCTCTTCCTAGGCCGTTTCTC-3' | |
NLS4 | forward | 5'-CATGG AGGCAGAGAGGCAGAGGAGGGAGAAGTTGA-3' |
reverse | 5'-CTAGT CAACTTCTCCCTCCTCTGCCTCTCTGCCTC-3' | |