Skip to main content

Table 3 Spearman correlations between the regular and high-sensitivity assays a, NSHDS and clinical research study subjects combined (n = 54 samples)

From: Temporal reliability of cytokines and growth factors in EDTA plasma

Biomarker LLD (pg/mL) % of samples above LLD % of samples above LLD that required extrapolationb n samples used to compute rs Spearman correlation coefficient (rs) rs p-value
  Regular assay High-sensitivity assay Regular assay (%) High-sensitivity assay (%) Regular assay (%) High-sensitivity assay (%)    
IL-1β 15 0.06 41 100 41 0 22 0.17 0.45
IL-2 6 0.16 52 100 64 0 28 0.18 0.36
IL-4 5 0.13 100 100 0 0 54 0.06 0.65
IL-5 3 0.01 0 100 N/A 2 N/A N/A N/A
IL-6 3 0.10 35 100 68 0 19 0.35 0.13
IL-7 10 0.12 50 98 93 0 27 0.11 0.59
IL-8 3 0.11 100 100 26 0 54 0.45 0.0007
IL-10 5 0.15 83 100 91 0 45 0.23 0.13
IL-13 10 0.48 98 100 81 0 53 -0.13 0.37
TNFα 10 0.05 100 100 66 0 54 0.15 0.28
IFNγ 5 0.29 100 100 0 0 54 0.08 0.56
  1. Note: All available plasma samples were used for these analyses, i.e., a maximum of 36 samples for the NSHDS and 18 samples for the clinical research subjects.
  2. a Regular assay kit from Biosource and high-sensitivity (hs) assay kit from Linco/Millipore
  3. b Values were extrapolated beyond the lowest point on the standard curve if their florescence intensity reading was above background florescence intensity. If florescence intensity was less than background florescence intensity, values were considered to be below the lower limit of detection.