Stage-specific fluorescence of GFP and mCherry. The stage specific fluorescence of RNA polymerase subunits β and β' during sporulation. Panels A, G, M and S are phase contrast images, Panels B, H, N and T are DAPI stained DNA images, Panels C, I, O and U are the β-GFP images, Panels D, J, P and V are the β'-mCherry images, Panels E, K, Q and W represent image overlays of the respective β-GFP and β'-mCherry images, and Panels F, L, R and × are linescans taken through the respective image overlays with green lines representing β-GFP and red lines representing β'-mCherry. The linescan in panel L is taken through the two asymmetrically dividing cells on the right of panel K. The lane scan in panel X is taken through the two cells with phase bright spores in panel W. The white arrows in the micrographs correspond to the black arrow in the respective linescan. The asterisks in the T6 micrographs are discussed in detail in the text. Fluorescence is in arbitrary units. Scale bar is 2 μm.