From: Stage-specific fluorescence intensity of GFP and mCherry during sporulation In Bacillus Subtilis
Plasmid | Genotype | Source/Construction |
---|---|---|
pYG1 | P spac - LIC-gfpmut3-erm | [8] |
pEU2 | P spac -'rpoB-gfpmut3-erm | This work |
pETMCSIII | bla P spac -10-6HisTφ | [17] |
pEU13 | P spac -'yloH-gfpmut3-erm | This work |
pEU14 | P spac -'ykzG-gfpmut3-erm | [8] |
pEU16 | P spac -'rpoE-gfpmut3-erm | [8] |
pEU21 | P spac -'rpoC-gfpmut3-erm | [8] |
pEU37 | P spac -'nusA-gfpmut3-erm | This work |
pNG583 | bla P spac -10-gfpmut3-6HisTφ | [8] |
pNG621 | P xyl - MCS-mCherry-cat | [8] |
pNG622 | P xyl -'rpoC-mCherry-cat | [8] |
pNG670 | P xyl -'nusA-mCherry-cat | This work |
pNG677 | P xyl -'ykzG-mCherry-cat | This work |
pNG735 | bla P spac -10-mCherry-6HisTφ | This work |
Strain | Genotype | Source/Construction |
E. coli | Â | Â |
BL21(DE3) pLysS | λ DE3 pLysS; F-ompT{lon}hsdSB (rB--mB-) | [18] |
DH5α | F- endAI hsdR17 supE44 thi-1 λ- recAI gyrA96 relA1 Δ(lacZY A-argF) U169 Φ80 dlacZ Δμ15 | Gibco BRL |
B. subtilis | Â | Â |
168trp+ | trpC2 chr::trp | [4] |
EU1 | 168trp+ chr:: erm P wt rpoB-gfp, P spac 'rpoB | This work: 168trp+ transformed with pEU2 |
EU16 | 168trp+ chr:: erm P wt yloH-gfp, P spac 'yloH | This work: 168trp+ transformed with pEU13 |
EU17 | 168trp+ chr:: erm P wt ykzG-gfp P spac 'ykzG | This work: 168trp+ transformed with pEU14 |
EU19 | 168trp+ chr:: erm P wt rpoE-gfp, P spac 'rpoE | This work: 168trp+ transformed with pEU16 |
EU44 | 168trp+ chr:: erm P wt rpoC-gfp, P spac 'rpoC | [8] |
EU49 | 168trp+ chr:: erm P wt nusA-gfp, P xyl 'nusA | This work: 168trp+ transformed with pEU37 |
EU128 | 168trp+ chr:: erm P wt rpoC-mCherrry, P xyl 'rpoC | This work: 168trp+ transformed with pNG621 |
EU131 | 168trp+ chr:: erm P wt ykzG-mCherrry, P xyl 'ykzG | This work: 168trp+ transformed with pNG677 |
EU142 | 168trp+ chr:: erm P wt nusA-mCherrry, P xyl 'nusA | This work: 168trp+ transformed with pNG670 |
EU156 | 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt rpoB-gfp, P spac 'rpoB | This work: EU126 transformed with pEU2 |
EU163 | 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt yloH-gfp, P spac 'yloH | This work: EU126 transformed with pEU13 |
EU164 | 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt ykzG-gfp, P spac 'ykzG | This work: EU126 transformed with pEU14 |
EU166 | 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt rpoE-gfp, P spac 'rpoE | This work: EU126 transformed with pEU16 |
EU183 | 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt nusA-gfp, P spac 'nusA | This work: EU126 transformed with pEU37 |
EU186 | 168trp+ chr:: cat P wt nusA-mCherrry, P xyl 'nusA, erm P wt ykzG-gfp, P spac 'ykzG | This work: EU142 transformed with pEU14 |
EU224 | 168trp+ chr:: cat P wt ykzG-mCherrry, P xyl 'ykzG, erm P wt nusA-gfp, P spac 'nusA | This work: EU131 transformed with pEU37 |
EU230 | 168trp+ chr:: cat P wt ykzG-mCherrry, P xyl 'ykzG, erm P wt rpoC-gfp, P spac 'rpoC | This work: EU131 transformed with pEU21 |