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Table 1 Plasmids and strains used in this work

From: Stage-specific fluorescence intensity of GFP and mCherry during sporulation In Bacillus Subtilis

Plasmid Genotype Source/Construction
pYG1 P spac - LIC-gfpmut3-erm [8]
pEU2 P spac -'rpoB-gfpmut3-erm This work
pETMCSIII bla P spac -10-6HisTφ [17]
pEU13 P spac -'yloH-gfpmut3-erm This work
pEU14 P spac -'ykzG-gfpmut3-erm [8]
pEU16 P spac -'rpoE-gfpmut3-erm [8]
pEU21 P spac -'rpoC-gfpmut3-erm [8]
pEU37 P spac -'nusA-gfpmut3-erm This work
pNG583 bla P spac -10-gfpmut3-6HisTφ [8]
pNG621 P xyl - MCS-mCherry-cat [8]
pNG622 P xyl -'rpoC-mCherry-cat [8]
pNG670 P xyl -'nusA-mCherry-cat This work
pNG677 P xyl -'ykzG-mCherry-cat This work
pNG735 bla P spac -10-mCherry-6HisTφ This work
Strain Genotype Source/Construction
E. coli   
BL21(DE3) pLysS λ DE3 pLysS; F-ompT{lon}hsdSB (rB--mB-) [18]
DH5α F- endAI hsdR17 supE44 thi-1 λ- recAI gyrA96 relA1 Δ(lacZY A-argF) U169 Φ80 dlacZ Δμ15 Gibco BRL
B. subtilis   
168trp+ trpC2 chr::trp [4]
EU1 168trp+ chr:: erm P wt rpoB-gfp, P spac 'rpoB This work: 168trp+ transformed with pEU2
EU16 168trp+ chr:: erm P wt yloH-gfp, P spac 'yloH This work: 168trp+ transformed with pEU13
EU17 168trp+ chr:: erm P wt ykzG-gfp P spac 'ykzG This work: 168trp+ transformed with pEU14
EU19 168trp+ chr:: erm P wt rpoE-gfp, P spac 'rpoE This work: 168trp+ transformed with pEU16
EU44 168trp+ chr:: erm P wt rpoC-gfp, P spac 'rpoC [8]
EU49 168trp+ chr:: erm P wt nusA-gfp, P xyl 'nusA This work: 168trp+ transformed with pEU37
EU128 168trp+ chr:: erm P wt rpoC-mCherrry, P xyl 'rpoC This work: 168trp+ transformed with pNG621
EU131 168trp+ chr:: erm P wt ykzG-mCherrry, P xyl 'ykzG This work: 168trp+ transformed with pNG677
EU142 168trp+ chr:: erm P wt nusA-mCherrry, P xyl 'nusA This work: 168trp+ transformed with pNG670
EU156 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt rpoB-gfp, P spac 'rpoB This work: EU126 transformed with pEU2
EU163 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt yloH-gfp, P spac 'yloH This work: EU126 transformed with pEU13
EU164 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt ykzG-gfp, P spac 'ykzG This work: EU126 transformed with pEU14
EU166 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt rpoE-gfp, P spac 'rpoE This work: EU126 transformed with pEU16
EU183 168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt nusA-gfp, P spac 'nusA This work: EU126 transformed with pEU37
EU186 168trp+ chr:: cat P wt nusA-mCherrry, P xyl 'nusA, erm P wt ykzG-gfp, P spac 'ykzG This work: EU142 transformed with pEU14
EU224 168trp+ chr:: cat P wt ykzG-mCherrry, P xyl 'ykzG, erm P wt nusA-gfp, P spac 'nusA This work: EU131 transformed with pEU37
EU230 168trp+ chr:: cat P wt ykzG-mCherrry, P xyl 'ykzG, erm P wt rpoC-gfp, P spac 'rpoC This work: EU131 transformed with pEU21