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Table 1 Plasmids and strains used in this work

From: Stage-specific fluorescence intensity of GFP and mCherry during sporulation In Bacillus Subtilis

Plasmid

Genotype

Source/Construction

pYG1

P spac - LIC-gfpmut3-erm

[8]

pEU2

P spac -'rpoB-gfpmut3-erm

This work

pETMCSIII

bla P spac -10-6HisTφ

[17]

pEU13

P spac -'yloH-gfpmut3-erm

This work

pEU14

P spac -'ykzG-gfpmut3-erm

[8]

pEU16

P spac -'rpoE-gfpmut3-erm

[8]

pEU21

P spac -'rpoC-gfpmut3-erm

[8]

pEU37

P spac -'nusA-gfpmut3-erm

This work

pNG583

bla P spac -10-gfpmut3-6HisTφ

[8]

pNG621

P xyl - MCS-mCherry-cat

[8]

pNG622

P xyl -'rpoC-mCherry-cat

[8]

pNG670

P xyl -'nusA-mCherry-cat

This work

pNG677

P xyl -'ykzG-mCherry-cat

This work

pNG735

bla P spac -10-mCherry-6HisTφ

This work

Strain

Genotype

Source/Construction

E. coli

  

BL21(DE3) pLysS

λ DE3 pLysS; F-ompT{lon}hsdSB (rB--mB-)

[18]

DH5α

F- endAI hsdR17 supE44 thi-1 λ- recAI gyrA96 relA1 Δ(lacZY A-argF) U169 Φ80 dlacZ Δμ15

Gibco BRL

B. subtilis

  

168trp+

trpC2 chr::trp

[4]

EU1

168trp+ chr:: erm P wt rpoB-gfp, P spac 'rpoB

This work: 168trp+ transformed with pEU2

EU16

168trp+ chr:: erm P wt yloH-gfp, P spac 'yloH

This work: 168trp+ transformed with pEU13

EU17

168trp+ chr:: erm P wt ykzG-gfp P spac 'ykzG

This work: 168trp+ transformed with pEU14

EU19

168trp+ chr:: erm P wt rpoE-gfp, P spac 'rpoE

This work: 168trp+ transformed with pEU16

EU44

168trp+ chr:: erm P wt rpoC-gfp, P spac 'rpoC

[8]

EU49

168trp+ chr:: erm P wt nusA-gfp, P xyl 'nusA

This work: 168trp+ transformed with pEU37

EU128

168trp+ chr:: erm P wt rpoC-mCherrry, P xyl 'rpoC

This work: 168trp+ transformed with pNG621

EU131

168trp+ chr:: erm P wt ykzG-mCherrry, P xyl 'ykzG

This work: 168trp+ transformed with pNG677

EU142

168trp+ chr:: erm P wt nusA-mCherrry, P xyl 'nusA

This work: 168trp+ transformed with pNG670

EU156

168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt rpoB-gfp, P spac 'rpoB

This work: EU126 transformed with pEU2

EU163

168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt yloH-gfp, P spac 'yloH

This work: EU126 transformed with pEU13

EU164

168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt ykzG-gfp, P spac 'ykzG

This work: EU126 transformed with pEU14

EU166

168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt rpoE-gfp, P spac 'rpoE

This work: EU126 transformed with pEU16

EU183

168trp+ chr:: cat P wt rpoC-mCherrry, P xyl 'rpoC, erm P wt nusA-gfp, P spac 'nusA

This work: EU126 transformed with pEU37

EU186

168trp+ chr:: cat P wt nusA-mCherrry, P xyl 'nusA, erm P wt ykzG-gfp, P spac 'ykzG

This work: EU142 transformed with pEU14

EU224

168trp+ chr:: cat P wt ykzG-mCherrry, P xyl 'ykzG, erm P wt nusA-gfp, P spac 'nusA

This work: EU131 transformed with pEU37

EU230

168trp+ chr:: cat P wt ykzG-mCherrry, P xyl 'ykzG, erm P wt rpoC-gfp, P spac 'rpoC

This work: EU131 transformed with pEU21