Table 2 Primers used in this work
From: Stage-specific fluorescence intensity of GFP and mCherry during sporulation In Bacillus Subtilis
| Plasmid | Primer Sequence (5'-3') |
|---|---|
| pEU2 |
rpoB F GGGTTCCTGGCGCGAGCGCAGCAGCCTCTTGGCGGTAAAGCGCAATTTGG rpoB R TTGGGCTGGCGCGAGCTTCTTTTGTTACTACATCGCGTTCAACGTCTGC |
| pEU13 |
YloH F GGGTTCCTGGCGCGAGCTTAGATCCGTCAATTGATTCTTTAATG YloH R TTGGGCTGGCGCGAGCTTCGCGGTCTTCCTTTTCAAACG |
| pEU14 |
YkzG F GGGTTCCTGGCGCGAGCATTTATAAGGTATTTTATCAAGAGAAGGCTG YkzG R TTGGGCTGGCGCGAGCTAACTCCAATACTTTAAAATTTTCGCTCTG |
| pEU16 |
RpoE F GGGTTCCTGGCGCGAGCCGCATCTTTGCTCGGCGTG RpoE R TTGGGCTGGCGCGAGCTTTAATTTCCTCTTCTTCATCATCATAGTC |
| pEU21 |
RpoC F GGGTTCCTGGCGCGAGCCGTAGAAGTAATGGTTCGCCAG RpoC R TTGGGCTGGCGCGAGCTTCAACCGGGACCATATCGTC |
| pEU37 |
NusA F GGGTTCCTGGCGCGAGCCACAGATGATCCTGACGTTGATC NusA R TTGGGCTGGCGCGAGCTTCATCCGATTCAGCAGTTTCAGG |
| pNG670 |
NusA mCherry F AAGGGGGGAGACCTCGAG ATGAGCAGTGAA (Xho I) NusA mCherry R ACCTAAAGTCACGAATTC TTCATCCGATTC (EcoR I) |
| pNG677 |
YkzG mCherry F AGATTTGGTACC ATTTATAAGGT (Acc 651) YkzG mCherry R TCATACCTCGAG TAACTCCAATA (Xho I) |
| pNG735 |
pETmCherry F TTTTTTCATATG GTGAGCAAGGGCGAGG (Nde I) pETmCherry R TTTTTTGAATTC CTACTTGTACAGCTCGTCC (EcoR I) |