Skip to main content
Figure 2 | BMC Research Notes

Figure 2

From: Identification of 5 novel germline APC mutations and characterization of clinical phenotypes in Japanese patients with classical and attenuated familial adenomatous polyposis

Figure 2

RT-PCR analysis for evaluation of the effect of the c.1958G > C mutation on splicing. RT-PCR with a forward primer for the sequence on exon 13 and a reverse primer for the sequence on exon 15 and subsequent agarose gel electrophoresis were performed on blood samples derived from two control subjects with the c.1958G/G genotype (lanes 1 and 2) and a patient with the c.1958G/C genotype (lane 3). The two main bands identified are indicated as band A (upper) and band B (lower). The intensity ratio (intensity of band B/intensity of band A) calculated with ImageJ software is shown under each lane of the panel. dw: distilled water. M: 100-bp ladder.

Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us