Figure 1
From: Methods for genetic manipulation of Burkholderia gladioli pathovar cocovenenans
mini-Tn 7 insertion site in BGC. A) The mini-Tn7 insertion site in BGC strain ATCC33664 determined from rescued plasmids. The sequence shown encompasses the last five codons of a putative glmS gene and its immediate downstream sequence, including the mini-Tn7 insertion sites 24 and 25 nucleotides downstream of the glmS stop codon. B) Presence of a single insertion site in BGC. Boiled cell lysates were prepared from six gentamicin resistant transformants obtained after transposition of mini-Tn7 T-Gm-REP into BGC. Chromosomal DNA contained in boiling preparation lysates was used as a template in PCR reactions containing PTn7 Land PTn7 BGC. The lane labeled M contained Hi-Lo molecular weight markers from Minnesota Molecular (Minneapolis, MN). All other lanes contain PCR products from six gentamicin resistant transformants.