Table 3 Restriction Fragment Length Polymorphism Assays
From: Association analysis of PRNP gene region with chronic wasting disease in Rocky Mountain elk
Sequence variant | Reagent Role | Reagent | Annealing Temp |
|---|---|---|---|
g.598T > C2 | Primer | CGAACATGCTTGTCATTCAGT | 58 |
| Â | Primer | ATTTATGGCCTGTCTGGAGGA | Â |
| Â | Enzyme | Bcl I | Â |
g.17918T > G2 | Primer | GCAAAATCTTGCCTGTTTCC | 58 |
| Â | Primer | GGGGAGGAGAAGAGGATCAC | Â |
| Â | Enzyme | Nsi I | Â |
g.18059C > T2 | Primer | GCAAAATCTTGCCTGTTTCC | 58 |
| Â | Primer | GGGGAGGAGAAGAGGATCAC | Â |
| Â | Enzyme | Bsa HI | Â |
g.3C > T3 | Primer | CCACAGAGCCACAGAGCTGCC | 63.7 |
| Â | Primer | GGGCTGGGTGAATTGTGTCATCCT | Â |
| Â | Enzyme | Hinc II | Â |
g.1477C > T2 | Primer | GGAGCCTCTTGCCCATAACA | 63 |
| Â | Primer | CTACTACCCTGGATTGAGCG | Â |
| Â | Enzyme | Rsa I | Â |
g.1591T > C2 | Primer | CAAATTTGGGTAATGATGTA | 56 |
| Â | Primer | TTCTACTACCCTGGATTGAG | Â |
| Â | Enzyme | Mae III | Â |
g.918A > G2 | Primer | CCTGTCGGAGTGTAACCTTGGGC | 63 |
| Â | Primer | TGAGGGAGCTGAGAGGCCAGG | Â |
| Â | Enzyme | Bcc I | Â |