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Figure 1 | BMC Research Notes

Figure 1

From: FONZIE: An optimized pipeline for minisatellite marker discovery and primer design from large sequence data sets

Figure 1

The graphical user interface of FONZIE. The graphical user interface is composed of five major sections. a. TRF parameters: All the modifiable parameters of TRF are located in this section. Users can modify each of them separately. b. Screening parameters. Users can modify the core motif size and the percent match between core motifs c. BLAST cut-off parameters: Users can modify the two E-value cut-off used during the screening for marker specificity and the virtual PCR steps d. Flanking sequences size and Primer3 parameters. e. The file field: In this section, users can open the Fasta- or Multifasta formatted sequence file, the BLAST database file, and the optional gff format file which contains the excluded regions.

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BMC Research Notes

ISSN: 1756-0500

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