Figure 3

Fluorescence of Cy2 is not affected in RNAlater. (A & B) FACS results. All data in FACS figures are restricted to cells defined by forward and side light scatter and further to singlet events. Dissociated cells were fixed and immunostained. The immunostained cells were visualized with secondary antibody conjugated with Cy2. The Cy2 positive cells are shown enclosed by red lines and negative cells are shown enclosed by black lines. (A) Cells in BSA: a population of Cy2 positive cells is clearly distinguished from Cy2 negative cells. (B) Cells in RNAlater: the two populations are distinguishable as well.