Table 2 PCR primers used for amplification of virulence genes
From: Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing
Target gene | Primer designation | Primer sequence (5'-3') | Amplicon size (bp) | Reference |
|---|---|---|---|---|
stx1 | stx1F | ATAAATCGCCATTCGTTGACTAC | 180 | (26) |
| Â | stx1R | AGAACGCCCACTGAGATCATC | Â | Â |
stx2 | stx2F | GGCACTGTCTGAAACTGCTCC | 255 | Â |
| Â | stx2R | TCGCCAGTTATCTGACATTCTG | Â | Â |
eaeA | eaeAF | GACCCGGCACAAGCATAAGC | 384 | Â |
| Â | eaeAR | CCACCTGCAGCAACAAGAGG | Â | Â |
hlyA | hlyAF | GCATCATCAAGCGTACGTTCC | 534 | Â |
| Â | hlyAR | AATGAGCCAAGCTGGTTAAGCT | Â | Â |
bfpA | bfpA | AATGGTGCTTGCGCTTGCTGC | 326 | (9) |
| Â | bfpR | GCCGCTTTATCCAACCTGGTA | Â | Â |