From: Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing
Gene | Primer sequencea | Reaction parametersb | Amplicon size (bp) | Gene ID | Locus tag |
---|---|---|---|---|---|
arcA | F: 5'-GAAGACGAGTTGGTAACACG-3' R: 5'-CTTCCAGATCACCGCAGAAGC-3' | 95°C for 1 min, 55°C for 2 min, 72°C for 3 min, 30 cycles | 680 | 959654 | Z6004 |
aroE | F: 5'-AAGGTGCGAATGTGACGGTG-3' R: 5'-AACTGGTTCTACGTCAGGCA-3' | 95°C for 1 min, 57°C for 2 min, 72°C for 3 min, 28 cycles | 620 | 961663 | Z2720 |
dnaE | F: 5'-GAG/TATGTGTGAGCTGTTTGC-3' R: 5'-CGA/GATA/CACCGCTTTCGCCG-3' | 94°C for 45 s, 45°C for 45 s, 72°C for 1 min, 30 cycles | 550 | 956917 | Z0196 |
mdh | F: 5'-CAACTGCCTTCAGGTTCAGAA-3' R: 5'-GCGTTCTGGATGCGTTTGGT-3' | 94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 30 cycles | 580 | 958670 | Z4595 |
gnd | F: 5'-GGCTTTAACTTCATCGGTAC-3' R: 5'-TCGCCGTAGTTCAGATCCCA-3' | 94°C for 45 s, 50°C for 45 s, 72°C for 1 min 10 s, 30 cycles | 590 | 962087 | Z3191 |
gapA | F: 5'-GATTACATGGCATACATGCTG-3' R: 5'-CAGACGAACGGTCAGGTCAAC-3' | 94°C for 45 s, 50°C for 45 s, 72°C for 1 min 10 s, 30 cycles | 535 | 961753 | Z2818 |
pgm | F: 5'-CCGTCCCATAACCCGCCGGAAG ATGGTGGTATCAAGTACAATCCG-3' R: 5'-TTACGCGTTTTTCAGAACTTCGCT AACAATCTCAACCGCTTCTTT-3' | 94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 35 cycles | 1146 | 957767 | Z0837 |
espA | F: 5'-ATGGATACATCAAATGCAACATC CGTTGTTAATGTGAGTGCGAGT-3' R: 5'-TTATTTACCAAGGGATATTGCTG AAATAGTTCTATATTGTAGAGA-3' | 94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 35 cycles | 579 | 960865 | Z5107 |
ompA | F: 5'-AGACAGCTATCGCGATTGC-3' R: 5'-GCTTTGTTGAAGTTGAACAC-3' | 94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 30 cycles | 691 | 958948 | Z1307 |