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Table 1 H. pylori genotyping

From: Application of PCR amplicon sequencing using a single primer pair in PCR amplification to assess variations in Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs

Subject No.

Gastritis classificationa

16S rDNAb type

cag-PAI

PCR analysis

CagA

EPIYA types

   

ESc

cagE

 

6

P-2-na

"Strain A"

-

+

AB

9

A-I-a

J99

-

+

ABC

12

C-3-a

26695

-

+

ABC + ABCC

14

A-I-a

26695

-

+

ABC

18

P-I-na

26695

-

+

ABC

21

P-I-na

J99

+

-

-

22

C-I-a

J99

-

+

AB

23

P-2-na

26695

-

+

ABC

25

P-2-na

26695

-

+

ABC

27

A-I-a

26695

-

+

ABC + AB

28

A-I-na

26695/J99

-

-

-

reference strain

HP 26695

-

+

ABC

reference strain

HP J99

-

+

BC

reference strain

ATCC 43509T

-

+

ABCCC

  1. a) Gastritis classification according to the revised Sydney system [24]. A: antrum predominant gastritis; P: pangastritis; C: corpus dominant gastritis; I: mild degree; 2: moderate degree; 3: severe degree; a: atrophy; na: no atrophy
  2. b) 16S rDNA variable V3 region motifs established by pyrosequencing analysis [19].
  3. c) ES: cag-PAI empty-site, + presence of a 380 bp amplicon, indicating loss of cag-PAI; - presence of cag-PAI [6].