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Table 1 H. pylori genotyping

From: Application of PCR amplicon sequencing using a single primer pair in PCR amplification to assess variations in Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs

Subject No. Gastritis classificationa 16S rDNAb type cag-PAI
PCR analysis
CagA
EPIYA types
    ESc cagE  
6 P-2-na "Strain A" - + AB
9 A-I-a J99 - + ABC
12 C-3-a 26695 - + ABC + ABCC
14 A-I-a 26695 - + ABC
18 P-I-na 26695 - + ABC
21 P-I-na J99 + - -
22 C-I-a J99 - + AB
23 P-2-na 26695 - + ABC
25 P-2-na 26695 - + ABC
27 A-I-a 26695 - + ABC + AB
28 A-I-na 26695/J99 - - -
reference strain HP 26695 - + ABC
reference strain HP J99 - + BC
reference strain ATCC 43509T - + ABCCC
  1. a) Gastritis classification according to the revised Sydney system [24]. A: antrum predominant gastritis; P: pangastritis; C: corpus dominant gastritis; I: mild degree; 2: moderate degree; 3: severe degree; a: atrophy; na: no atrophy
  2. b) 16S rDNA variable V3 region motifs established by pyrosequencing analysis [19].
  3. c) ES: cag-PAI empty-site, + presence of a 380 bp amplicon, indicating loss of cag-PAI; - presence of cag-PAI [6].