BAC deletions generated with lox66 transposons pTnLox66(B)markerless and pTnLox66(B)markerless Enhancer-Trap sequenced with Primers Seq 4-compliment and LF8-compliment respectively. Top Panel: Sequence of BAC-C deletions 3, 4, 5, is shown. The four restriction enzyme sites (4 RE sites) indicated in the schematic representation are Not I, Asc I, Pme I and Pac I, and correspond to sites in pTnLox66(B)markerless transposon shown in Figure 2 top panel. These sites are highlighted by different colors in the sequence presented. Bottom Panel: Sequence of BAC-D deletions 7-11 obtained with pTnLox66(B)markerless Enhancer-Trap transposon is shown. The Pac I site is highlighted in color. Sequence complimentary to the lox66 site is colored and underlined in both panels. Sequencing in the opposite direction with transposon end-based primer Seq 1 in deletion clones 3-5 and 7-11 indicates zebrafish DNA which BLASTs to chromosome 9 (not shown). The locations of these sequences on Chr 9 are consistent with end-points of the BAC deletions expected from their sizes on the FIGE gel shown in Figure 3.