Skip to main content
Figure 5 | BMC Research Notes

Figure 5

From: Replacing the wild type loxP site in BACs from the public domain with lox66 using a lox66 transposon

Figure 5

A schematic representation of the three Cre-recombination steps in the end-deletion/ lox66 substitution process. Step 1 shows the end-deletion of genomic insert DNA by the transposed lox66 site recombining with the loxP endogenous to the BAC clone. Step 2 illustrates co-integrate formation between lox66 APPb BAC and P1 phage DNA carrying loxP. Step 3 shows regeneration of circular lox66 BAC from the linear DNA in the phage head after infection of fresh bacteria. The lox511 site at the other end of genomic insert DNA is omitted for clarity purposes, as it does not play any role in Cre recombinations with either lox66 or loxP. Note that the relative position of the two lox sites in the linear DNA inside the phage head determines whether lox66 or loxP is retained in the BAC. This in turn is determined by the location of the "pac site" in the co-integrate shown in Step 2 (see [16, 20], for detailed discussion).

Back to article page