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Table 1 Quantity of nucleic acids extracts obtained by the respective protocols

From: Evaluation of cost-effective total nucleic acids extraction protocols for cultured Mycobacterium tuberculosis; a comparison by PCR amplification of genes associated with drug resistance

  Protocol 1 Protocol 2 Protocol 3 Protocol 4 Protocol 5 Protocol 6 Protocol 7
Relative amount of nucleic acids 1 ~2 >2 4 4 8 2
Amount of nucleic acid (μg) 15.7 ± 3.2 28.8 ± 6.7 36.9 ± 15.5 68.4 ± 22.7 70.4 ± 20.3 133.7 ± 8.9 32.5 ± 2.4
Temp' Treatments heating heating freezing and heating heating heating heating heating
Chemical Treatments   SDS   Tris/EDTA Tris/EDTA/SDS Tris/EDTA/Triton X-100 Tris/EDTA/Tween 20
Ratio of UV absorbance (A 260/280 nm ) 0.20 1.18 1.23 1.25 1.39 0.73 1.27