Table 1 Quantity of nucleic acids extracts obtained by the respective protocols
Protocol 1 | Protocol 2 | Protocol 3 | Protocol 4 | Protocol 5 | Protocol 6 | Protocol 7 | |
|---|---|---|---|---|---|---|---|
Relative amount of nucleic acids | 1 | ~2 | >2 | 4 | 4 | 8 | 2 |
Amount of nucleic acid (μg) | 15.7 ± 3.2 | 28.8 ± 6.7 | 36.9 ± 15.5 | 68.4 ± 22.7 | 70.4 ± 20.3 | 133.7 ± 8.9 | 32.5 ± 2.4 |
Temp' Treatments | heating | heating | freezing and heating | heating | heating | heating | heating |
Chemical Treatments | SDS | Tris/EDTA | Tris/EDTA/SDS | Tris/EDTA/Triton X-100 | Tris/EDTA/Tween 20 | ||
Ratio of UV absorbance (A 260/280 nm ) | 0.20 | 1.18 | 1.23 | 1.25 | 1.39 | 0.73 | 1.27 |