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Table 2 Amplification of selected genes for each the seven protocols evaluated

From: Evaluation of cost-effective total nucleic acids extraction protocols for cultured Mycobacterium tuberculosis; a comparison by PCR amplification of genes associated with drug resistance

Genes amplified Protocol 1 Protocol 2 Protocol 3 Protocol 4 Protocol 5 Protocol 6 Protocol 7
rpo B X X X - -
Kat G X X X X - -
rrs X X - -
Treatments        
Temperature heating heating freezing and heating heating heating heating heating
Chemical   SDS   Tris/EDTA Tris/EDTA/SDS Tris/EDTA/TritonX-100 Tris/EDTA/Tween20
  1. expected amplification; X no amplification; - inconsistent amplification