Evaluation of cost-effective total nucleic acids extraction protocols for cultured Mycobacterium tuberculosis; a comparison by PCR amplification of genes associated with drug resistance

Table 2 Amplification of selected genes for each the seven protocols evaluated

Genes amplified	Protocol 1	Protocol 2	Protocol 3	Protocol 4	Protocol 5	Protocol 6	Protocol 7
rpo B	X	X	√	√	X	-	-
Kat G	X	X	X	√	X	-	-
rrs	√	X	√	√	X	-	-
Treatments
Temperature	heating	heating	freezing and heating	heating	heating	heating	heating
Chemical		SDS		Tris/EDTA	Tris/EDTA/SDS	Tris/EDTA/TritonX-100	Tris/EDTA/Tween20

√ ⇒ expected amplification; X ⇒ no amplification; - ⇒ inconsistent amplification

ISSN: 1756-0500