Table 1 Bacterial strains and plasmids

Bacterial strains:

Escherichia coli

DB1317

recD1014 (Nuc^-)

CGSC

MS15978

DB1317 harbouring pMR1, Ap^r

This study

MS14395

DH5α, recA1, harbouring pMR2, Cm^r

[14], this study

Bacillus subtilis

Strain 168

1A700

BGSC

Streptococcus gordonii

LTH 5597

TIGR strain harbouring pMK110, Ery^r

[12]

Plasmids:

pMR1

pBR322 vector, nptIIΔNco I, Ap^r

[10]

pMR2

pACYC184 vector, nptII without promoter, Cm^r

[10]

pMK110

pMG36e vector, nptIIΔNco I from pMR1, Ery^r

[15]

pAW105

pUC19 vector, cat from pC194, Bacillus thuringiensis replicon, Ap^r

[16]

Plant:

Solanum tuberosum cv. Apriori

Genetically modified potato containing antisense GBSS (granule bound starch synthase) and intact nptII

AVEBE, Foxhole, The Netherlands