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Table 1 Bacterial strains and plasmids

From: Lack of detectable DNA uptake by transformation of selected recipients in mono-associated rats

Strains or plasmids Description Reference or source
Bacterial strains:   
Escherichia coli   
DB1317 recD1014 (Nuc-) CGSC
MS15978 DB1317 harbouring pMR1, Apr This study
MS14395 DH5α, recA1, harbouring pMR2, Cmr [14], this study
Bacillus subtilis   
Strain 168 1A700 BGSC
Streptococcus gordonii   
LTH 5597 TIGR strain harbouring pMK110, Eryr [12]
Plasmids:   
pMR1 pBR322 vector, nptIIΔNco I, Apr [10]
pMR2 pACYC184 vector, nptII without promoter, Cmr [10]
pMK110 pMG36e vector, nptIIΔNco I from pMR1, Eryr [15]
pAW105 pUC19 vector, cat from pC194, Bacillus thuringiensis replicon, Apr [16]
Plant:   
Solanum tuberosum cv. Apriori Genetically modified potato containing antisense GBSS (granule bound starch synthase) and intact nptII AVEBE, Foxhole, The Netherlands
  1. Abbreviations: Apr: ampicillin resistance; BGSC: Bacillus Genetic Stock Center; cat: chloramphenicol acetyltransferase gene; CGSC: E. coli Genetic Stock Center; Cmr: chloramphenicol resistance; Eryr: erythromycin resistance; GBSS: granule bound starch synthase gene;nptII: kanamycin resistance gene.