Figure 3
CTGF mRNA expression in PGE 2 -stimulated chondrocytes: blocking experiments. Chondrocyte samples (n = 5, in duplicate) were either stimulated by PGE2 (10 nM) or not for 24 h, with or without pretreatment with an EP-specific antagonist (AH: AH6809, 10 ng/ml; GW: GW627368X, 5 μM), for 1 h in vitro. The relative expression of CTGF/CCN2 mRNA was calculated against values of the internal control, GAPDH using quantitative PCR.