Phenotypic analyses of transgenic lines expressing pri-smiRNA(CHS). (A) Comparison of the seed coat color of seeds from different transgenic lines numbered 1-11 expressing pri-smiRNA(CHS). Wild type (wt) and chs knockout seeds (tt4) are shown for reference. (B) Thin layer chromatography of methanolic extracts from the same transgenic lines to analyze flavonol content. Green (asterisks), kaempferol glycosides; red (squares), quercetin glycosides; blue (dots), sinapate derivatives. Wild type plants were grown either without (wt-) or with 4% sucrose (wt+). All other plants were grown with 4% sucrose. (C) Relative anthocyanin content in seedlings of the same transgenic lines.