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Figure 4 | BMC Research Notes

Figure 4

From: Improvement of the design and generation of highly specific plant knockdown lines using primary synthetic microRNAs (pri-smiRNAs)

Figure 4

Analysis of off-target transcript levels. One potential off-target predicted with RNAhybrid was tested for smiRNA(CHS)-mediated effects on transcript abundance. RNA was extracted from different transgenic lines that highly over-expressed pri-smiRNA(CHS), and qRT-PCR experiments were performed to quantify the relative transcript levels of At1g40390. Data were normalized to wild type (wt) transcript levels. See also Figure 2B.

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