Skip to main content
Figure 3 | BMC Research Notes

Figure 3

From: An enhanced method for sequence walking and paralog mining: TOPO® Vector-Ligation PCR

Figure 3

Extension of sequence from clone 143-2-1 in the 5' (A) and 3' (B) directions. First round TVL-PCR products (top) were generated from templates prepared using Hin dIII (H), Eco RI (E), or Bam HI (B) digestion of genomic DNA. First round products were used as templates to generate second round products (bottom). Boxes (bottom gels) indicate the locations of the second round TVL-PCR products that proved to be the desired products.

Back to article page
\