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Table 1 Primer Information.

From: An enhanced method for sequence walking and paralog mining: TOPO® Vector-Ligation PCR

Primer Name Primer Sequence Tm (°C) *
Vector Primers   
M13F 5'-GTAAAACGACGGCCAG-3' 50.7
M13R 5'-CAGGAAACAGCTATGAC-3' 47.0
T3 5'-ATTAACCCTCACTAAAGGGA-3' 50.3
T7 5'-TAATACGACTCACTATAGGG-3' 47.5
Gene Primers   
14321 5' F1 5'-AGGGTTAGGTTTAAGGTTGAG-3' 51.8
14321 5' F2 5'-GTGAGGGTACGAAAGTAGG-3' 51.9
14321 3' F1 5'-TCGCAAGTTGAACTATGTATCC-3' 52.6
14321 3' F2 5'-CAGTTTGTTCAGGACTGAGT-3' 52.2
  1. Vector primer sequences are standard, and are provided here for convenience. Gene primers were designed by us.
  2. *Melting temperature values were determined by the Integrated DNA Technologies OligoAnalyzer application [7], and may not be the same as those published elsewhere for M13_ and T_ primers.