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Figure 4 | BMC Research Notes

Figure 4

From: Evidence that low endocytic activity is not directly responsible for human serum resistance in the insect form of African trypanosomes

Figure 4

T. brucei BSF parasites cultured under low glucose conditions possess reduced ATP levels. A. T. brucei BSF parasites were either cultured in RPMI-1640 media containing 0 mM or 25 mM D-glucose or 10 mM 2-deoxy-D-glucose, all supplemented with dialyzed (glucose-free) 10% FBS. A reduction in ATP was observed in parasites cultured in either 0 mM D-glucose or 10 mM 2-deoxy-D-glucose. Data are normalized to 100% for 25 mM D-glucose and are the mean of three independent assays. Error bars show the standard deviation. B. T. brucei BSF parasites were cultured in RPMI-1640 media containing 5 mM D-glucose and dialyzed (glucose-free) 10% FBS for twelve hours and ATP levels measured at intervals. Data are the mean of three independent determinations, and error bars show the standard deviation.

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