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Table 1 Effect of glucose deprivation on motility and morphology in bloodstream trypanosomes.

From: Evidence that low endocytic activity is not directly responsible for human serum resistance in the insect form of African trypanosomes

Treatment Conc (mM) 15 min 2 h 6 h 8 h 12 h
  morp mot morp mot morp mot morp mot morp mot
Control 25 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
2-deoxy-D-glucose 0.1 Dead Dead NA NA NA NA NA NA NA NA
  1.0 Dead Dead NA NA NA NA NA NA NA NA
  10 Dead Dead NA NA NA NA NA NA NA NA
D-Glucose 0.0 Dead Dead NA NA NA NA NA NA NA NA
  0.1 Dead Dead NA NA NA NA NA NA NA NA
  1.0 100% 50% Dead Dead NA NA NA NA NA NA
  5.0 100% 100% 90% 100% 65% 80% 55% 65% 20% 35%
  10 100% 100% 100% 100% 100% 100% 80% 90% 50% 50%
  1. Log-phase trypanosomes were harvested, cells washed in PBS and resuspended in HMI-9 medium supplemented with 2-deoxy-D-glucose, in standard formulation HMI-9 (25 mM) or glucose free RPMI-1640 supplemented with D-glucose as indicated. All media also contained dialyzed 10% fetal bovine serum. Cells were incubated at 37°C and observed under phase contrast at intervals. Aliquots were withdrawn at various times and mounted on pre-warmed microscope slides. Normal cells were scored as a length:breadth ratio of >5:1 while rounded cells scored with a ratio of 2:1 or less. "morp"; percent of cells retaining normal morphology. Abnormalities includes rounded cells or emergence of a BigEye flagellar pocket. "mot"; motility; cells were scored as motile if there was evidence of cell movement, including flagellar twitching. NA; not analyzed.