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Figure 1 | BMC Research Notes

Figure 1

From: Tousled kinase TLK1B mediates chromatin assembly in conjunction with Asf1 regardless of its kinase activity

Figure 1

TLK1B mediates chromatin assembly with Asf1 regardless of Asf1 phosphorylation. A. In vitro phosphorylation of Asf1 wt and S192Y mutant by TLK1B. B. Chromatin assembly on a plasmid is stimulated equally well by Asf1 wt and mutant, and the addition of wt TLK1B further stimulates plasmid supercoiling. C. Chromatin assembly is stimulated by TLK1B, wt or KD, and the addition of wt or mutant Asf1 synergizes in plasmid supercoiling. D. Western blot for Asf1a in nuclear extract supplemented or not with recombinant Asf1a. The blot was also probed for eIF4E (4E) as a loading control.

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