A new mutant genetic resource for tomato crop improvement by TILLING technology

BMC Research Notes

Table 3 Mutation density in 0.7% EMS and 1% EMS Red Setter populations

Target gene		No. of screened M3 families		No. of identified mutations		Overall mutation density
Name	Amplicon size (kb)	0.7% EMS	1% EMS	0.7% EMS	1% EMS	0.7% EMS	1% EMS
*Rab11a*	0.407	1,373	713	1	3	1/559 kb	1/97 kb
PG	2.587	2,791	963	7	2	1/1031 kb	1/1246 kb
*Exp1*	1.025	3,885	1,284	14	6	1/284 kb	1/219 kb
*RIN*	1.331	3,885	1,284	4	8	1/1293 kb	1/214 kb
Gr	1.409	3,885	1,284	5	3	1/1095 kb	1/603 kb
*Lcy-b*	1.274	3,801	1,252	4	3	1/1211 kb	1/532 kb
*Lcy-e*	1.414	3,630	1,185	6	0	1/855 kb	-
Total/mean	9.447			41	25	1/574 kb	1/322 kb

The accession numbers of the analyzed seven target genes are the following: Rab11a [GenBank:AJ245570], PG [GenBank:M37304], Exp1 [GenBank:AF548376], RIN [GenBank:AF448522], Gr [GenBank:DQ372897], Lcy-b [GenBank:CQ788383], Lcy-e [GenBank:Y14387]. The number of screened M3 families, the number of identified mutations and the overall mutation density, estimated as described in Methods, are reported both for 0.7% and 1% EMS Red Setter populations.

ISSN: 1756-0500