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Table 3 Mutation density in 0.7% EMS and 1% EMS Red Setter populations

From: A new mutant genetic resource for tomato crop improvement by TILLING technology

Target gene No. of screened M3 families No. of identified mutations Overall mutation density
Name Amplicon size (kb) 0.7% EMS 1% EMS 0.7% EMS 1% EMS 0.7% EMS 1% EMS
Rab11a 0.407 1,373 713 1 3 1/559 kb 1/97 kb
PG 2.587 2,791 963 7 2 1/1031 kb 1/1246 kb
Exp1 1.025 3,885 1,284 14 6 1/284 kb 1/219 kb
RIN 1.331 3,885 1,284 4 8 1/1293 kb 1/214 kb
Gr 1.409 3,885 1,284 5 3 1/1095 kb 1/603 kb
Lcy-b 1.274 3,801 1,252 4 3 1/1211 kb 1/532 kb
Lcy-e 1.414 3,630 1,185 6 0 1/855 kb -
Total/mean 9.447    41 25 1/574 kb 1/322 kb
  1. The accession numbers of the analyzed seven target genes are the following: Rab11a [GenBank:AJ245570], PG [GenBank:M37304], Exp1 [GenBank:AF548376], RIN [GenBank:AF448522], Gr [GenBank:DQ372897], Lcy-b [GenBank:CQ788383], Lcy-e [GenBank:Y14387]. The number of screened M3 families, the number of identified mutations and the overall mutation density, estimated as described in Methods, are reported both for 0.7% and 1% EMS Red Setter populations.